anti cb1r  (Proteintech)

Journal: European Journal of Medical Research

Article Title: Epigenetic regulation by WTAPP1 promotes trophoblast dysfunction in gestational diabetes via CNR1/NF-κB activation

doi: 10.1186/s40001-025-03217-8

Figure Lengend Snippet: WTAPP1 silence downregulated the expression of CNR1 and the NF-κB signaling pathway. A CNR1 mRNA expression was detected in GDM and NC populations in clinic (fold: relative to NC group). B The correlation between CNR1 mRNA and WTAPP1 expression in placental samples of GDM patients. C Western blot detection of CNR1, p-IkBα, IkBα, p-p65, p65, and nuclear p65. D RT-qPCR analysis of CNR1 mRNA expression in the cells transfected with kdWP1 and cultured with NG and HG (fold: relative to NG group). E Western blot detection of the protein expressions of CNR1 and the NF-κB signaling pathway in the cells transfected with kdWP1 and cultured with NG and HG. N = 3 independent experiments. ns P > 0.05, * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: CNR1 , Rabbit , Proteintech , 17978-1-AP , 1:2000.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Transfection, Cell Culture

Journal: European Journal of Medical Research

Article Title: Epigenetic regulation by WTAPP1 promotes trophoblast dysfunction in gestational diabetes via CNR1/NF-κB activation

doi: 10.1186/s40001-025-03217-8

Figure Lengend Snippet: WTAPP1 promoted CNR1 expression in an m6A-dependent manner. A Bioinformatics prediction of m6A enrichment in CNR1 mRNA sequence. B m6A enrichment levels of CNR1 mRNA in placental samples of GDM and NC group and in WTAPP1-knockdown cells treated with NG or HG (fold: relative to NC or NG group). C RT-qPCR analysis of WTAP mRNA expression in placental samples of GDM and NC group and in WTAPP1-knockdown cells treated with NG or HG (fold: relative to NC or NG group). D Western blot detection of WTAP protein levels in placental samples of GDM and NC group and in WTAPP1-knockdown cells treated with NG or HG. Cells were transfected with WTAPP1 overexpression vectors (oeWP1) and WTAP knockdown vectors (oeWP1/kdW). E The gene expression of WTAP1 and CNR1 was analyzed in cells (fold: relative to NC group). F The m6A enrichment of CNR1 mRNA (fold: relative to NC group). G The mRNA remaining of CNR1 in cells treated by actinomycin D (fold: relative to 0 h). H-I The protein expression of CNR1, p-IkBα, IkBα, p-p65, and p65. J, The protein expression of nuclear p65. ns P > 0.05, * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: CNR1 , Rabbit , Proteintech , 17978-1-AP , 1:2000.

Techniques: Expressing, Sequencing, Knockdown, Quantitative RT-PCR, Western Blot, Transfection, Over Expression, Gene Expression

Journal: European Journal of Medical Research

Article Title: Epigenetic regulation by WTAPP1 promotes trophoblast dysfunction in gestational diabetes via CNR1/NF-κB activation

doi: 10.1186/s40001-025-03217-8

Figure Lengend Snippet: Validation of the expression of the WTAPP1/CNR1/NF-κB signaling. A HTR-8/Svneo cells were transfected with WTAPP1 knockdown vectors (kdWP1), CNR1 overexpression vectors (oeC), and treated with NF-κB inhibitor BAY-11-7085 (Bay) or HG. The protein expression of WTAP, CNR1, p-IkBα, IkBα, p-p65, and p65 in cells was detected by western blot analysis. B The expression of nuclear p65 protein in cells was detected by western blot analysis. N = 3 independent experiments. ns P > 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: CNR1 , Rabbit , Proteintech , 17978-1-AP , 1:2000.

Techniques: Biomarker Discovery, Expressing, Transfection, Knockdown, Over Expression, Western Blot

Journal: European Journal of Medical Research

Article Title: Epigenetic regulation by WTAPP1 promotes trophoblast dysfunction in gestational diabetes via CNR1/NF-κB activation

doi: 10.1186/s40001-025-03217-8

Figure Lengend Snippet: Blocking the WTAPP1/CNR1/NF-κB signaling reduced high glucose-induced cellular damage in trophoblast cells. A Cell viability in the above five groups of HTR-8/Svneo cells. B Cell apoptosis detected by flow cytometry. C Western blot analysis of apoptosis-related proteins, including cleaved caspase-3, Bcl2, and Bax. D ELISA detection of inflammatory cytokines (including IL-1β, IL-6, TNF-α, and MCP-1). E The levels of oxidative stress markers in cells, including MDA, GSH/GSSG, CAT, and SOD. N = 3 independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: CNR1 , Rabbit , Proteintech , 17978-1-AP , 1:2000.

Techniques: Blocking Assay, Flow Cytometry, Western Blot, Enzyme-linked Immunosorbent Assay